Cell Reprogramming Laboratory

International University

Publications

  1. Thuy Van, N.T., Anh My, L.B., Van Thuan, N., & Bui, H.T. (2020). Improve the developmental competence of porcine oocytes from small antral follicles by pre-maturation culture method. Theriogenology, 149, 139-148 .    http://www.ncbi.nlm.nih.gov/pubmed/32272343
  2. Nguyen, H. H., Nhu, B., Uyen, N., Nguyen, V. T., & Bui, H. T. (2019). Isolation of female germline stem cells from porcine ovarian tissue and differentiation into oocyte-like cells. The Journal of reproduction and development, 65(5), 423–432.  http://www.ncbi.nlm.nih.gov/pubmed/31378755
  3. Ho, N., Nguyen, T., Nguyen, T. V., & Bui, H. T. (2019). Epigenetic impairments in development of parthenogenetic preimplantation mouse embryos. The Journal of reproduction and development, 65(1), 83–90.  http://www.ncbi.nlm.nih.gov/pubmed/30606958
  4. Hong-Thuy Bui,Nguyen Van Thuan, Deug-Nam Kwon, Yun-Jung Choi, Min-Hee Kang, Jae-Woong Han, Teoan Kim and Jin-Hoi Kim. Identification and characterization of putative stem cells in the adult pig ovary. Development, 141, May, 2014 (IF: 7.09).
    http://www.ncbi.nlm.nih.gov/pubmed/24866115
  5. Sayaka Wakayama, Takashi Kohda, Haruko Obokata, Mikiko Tokoro, Chong Li, Yukari Terashita, Eiji Mizutani, Van Thuan Nguyen, Satoshi Kishigami, Fumitoshi Ishino, TeruhikoWakayama. Successful serial recloning in the mouse over multiple generations. CELL (Cell Stem Cell),12:293-297, 2013.(IF: 23.494)
    http://www.ncbi.nlm.nih.gov/pubmed/23472871
  6. Hong-Thuy Bui, Deug-Nam Kwon, Min-Hui Kang, Mi-Hye Oh, Mi-Ryung Park, Woo-Jin Park, Seung-Sam Paik, Nguyen Van Thuan, Jin-Hoi Kim. Epigenetic reprogramming in somatic cells induced by extract from germinal vesicle stage pig oocytes. Development, 2012.(IF: 7.09)
    http://www.ncbi.nlm.nih.gov/pubmed/23132243
  7. Jeon K, Lim H, Kim JH,Van Thuan N, Park SH, Lim YM, Choi HY, Lee ER, Kim JH, Lee MS, Cho SG. Differentiation and transplantation of functional pancreatic beta cells generated from induced pluripotent stem cells derived from a type 1 diabetes mouse model. Stem Cells and Development. 2012 (IF: 4.46).
    http://www.ncbi.nlm.nih.gov/pubmed/22512788
  8. Bui HT and Van Thuan N. Histone Deacetylase Inhibition Improves Activation of Ribosomal RNA Genes and Embryonic Nucleolar Reprogramming in Cloned Mouse Embryos. Biol Reprod, 2011 (IF: 4.009).
    http://www.ncbi.nlm.nih.gov/pubmed/21753193.
  9. Park MR, Lee AR, Bui HT, Park C, Park KK, Cho SG, Song H, Kim JH, Van Thuan N, Kim JH. Chromosome remodeling and differentiation of tetraploid embryos during preimplantation development. Dev Dyn. 240:1660-1669, 2011 (IF: 3.018).
    http://www.ncbi.nlm.nih.gov/pubmed/21547981.
  10. Bui HT, Wakayama S, Mizutani E, Park KK, Kim JH, Van Thuan N, Wakayama T. Essential role of paternal chromatin in the regulation of transcriptional activity during mouse preimplantation development. Reproduction. 141:67-77, 2011(IF: 3.413).
    http://www.ncbi.nlm.nih.gov/pubmed/20974742 .
  11. Park JY, Park MR, Hwang KC, Chung JS, Bui HT, Kim T, Cho SK, Kim JH, Hwang S, Park SB,Van Thuan N, Kim JH. Comparative gene expression analysis of somatic cell nuclear transfer-derived cloned pigs with normal and abnormal umbilical cords. Biol Reprod. 84:189-199, 2011 (IF: 4.009).
    http://www.ncbi.nlm.nih.gov/pubmed/20864643
  12. Bui HT, Wakayama S, Kishigami S, Park KK, Kim JH,Van Thuan N, Wakayama T.  Effect of trichostatin A on chromatin remodeling, histone modifications, DNA replication, and transcriptional activity in cloned mouse embryos.Biol Reprod  83:454-463, 2010 (IF: 4.009).
    http://www.ncbi.nlm.nih.gov/pubmed/20505166 .
  13. Van Thuan N, Kishigami S, Wakayama T. How to improve the success rate of mouse cloning technology. Journal of Reproduction and Development. 2010 (IF: 1.46).
    http://www.ncbi.nlm.nih.gov/pubmed/20203432 .
  14. Van Thuan N, Hong-Thuy Bui, Takafusa Hikichi, Sayaka Wakayama, Satoshi Kishigami, Eiji Mizutani and Teruhiko Wakayama. Histone deacetylase inhibitor scriptaid rescues full-term development in cloned inbred mice by enhancing nascent mRNA production.Reproduction, 138: 309-317, 2009 (IF: 3.413).
    http://www.ncbi.nlm.nih.gov/pubmed/19433501
  15. Van Thuan N, Wakayama S, Kishigami S, Ohta H, Hikichi T, Mizutani E, Bui HT, Wakayama T. Injection of Somatic Cell Cytoplasm into Oocytes Before ICSI Impairs Full-Term Development and Increases Placental Weight in Mice.Biol Reprod. 74: 865–873, 2006 (IF: 4.009).
    http://www.ncbi.nlm.nih.gov/pubmed/16436529
  16. Van Thuan N, Wakayama S, Kishigami S, Wakayama T. Donor Centrosome Regulation of Initial Spindle Formation in Mouse Somatic Cell Nuclear Transfer: Roles of Gamma-Tubulin and Nuclear Mitotic Apparatus Protein 1.Biol Reprod. 74:777–787, 2006 (IF: 4.009).
    http://www.ncbi.nlm.nih.gov/pubmed/16407502
  17. Van Thuan N, Sayaka Wakayama, Satoshi Kishigami, and Teruhiko Wakayama. New Preservation Method for Mouse Spermatozoa Without Freezing.Biol Reprod. 72:444-450, 2005 (IF: 4.009).
    http://www.ncbi.nlm.nih.gov/pubmed/15483221 .
  18. Van Thuan N, Kure-bayashi S, Harayama H, Nagai T, Miyake M. Stage-Specific Effects of The Osmolarity of A Culture Medium on The Development of Parthenogenetic Diploids in The Pig.Theriogenology. 59:719-734, 2003 (IF: 2.274).
    http://www.ncbi.nlm.nih.gov/pubmed/12517376 .
  19. Van Thuan N, Hiroshi Harayama, Masashi Miyake. Characteristics of Preimplantational Development of Porcine Parthenogenetic Diploids Relative to the Existence of Amino Acids in Vitro.Biol Reprod. 67:1688-1698, 2002 (IF:4.009).
    http://www.ncbi.nlm.nih.gov/pubmed/12444042 .
  20. Van Thuan N, Hiroshi Harayama, Masashi Miyake. The Development of Porcine Parthenogenetic Diploid Oocytes with Homogeneous Genomic Components In Vitro.JReprod Dev. 48:157-165, 2002 (IF: 4.009).
    http://www.jstage.jst.go.jp/article/jrd/48/2/157/ .
  21. Bui HT, Hwang KC, Kim JH,Van Thuan N, Wakayama T, Miyano T. Effect of vanadate on the chromatin configuration in pig GV-oocytes. J Reprod Dev. 55:367-372. 2009 (IF: 1.46).
    http://www.ncbi.nlm.nih.gov/pubmed/19404002 .
  22. Cho SK, Hwang KC, Choi YJ, Bui HT,Van Thuan N, Park C, Kim JH, Kim JH. Production of transgenic pigs harboring the human erythropoietin (hEPO) gene using somatic cell nuclear transfer. J Reprod Dev. 55:128-136. 2009 (IF:1.46).
    http://www.ncbi.nlm.nih.gov/pubmed/19106487 .
  23. Binh NT,Van Thuan N, Miyake M. Effects of liquid preservation of sperm on their ability to activate oocytes and initiate preimplantational development after intracytoplasmic sperm injection in the pig.  71:1440-1450. 2009 (IF: 2.274).
    http://www.ncbi.nlm.nih.gov/pubmed/19324403 .
  24. Bui HT, Wakayama S, Kishigami S,Van Thuan N, Wakayama T. The cytoplasm of mouse germinal vesicle (GV) stage oocytes can enhance somatic cell nuclear reprogramming. Development, 135:3935-3945. 2008 (IF: 7.09).
    http://www.ncbi.nlm.nih.gov/pubmed/18997114 .
  25. Sayaka W, Satoshi K,Van Thuan N, Hiroshi O, Takafusa H, Eiji M, Thuy BH, Masashi M, Teruhiko W. Effect of volume of oocyte cytoplasm on embryo development after parthenogenetic activation, intracytoplasmic sperm injection, or somatic cell nuclear transfer. Zygote. 16:211-222. 2008 (IF: 1.337).
    http://www.ncbi.nlm.nih.gov/pubmed/18578946 .
  26. Bui HT,Van Thuan N, Kishigami S, Wakayama S, Hikichi T, Ohta H, Mizutani E, Yamaoka E, Wakayama T, Miyano T. Regulation of chromatin and chromosome morphology by histone H3 modifications in pig oocytes. Reproduction. 133:371-382. 2007 (IF: 3.413).
    http://www.ncbi.nlm.nih.gov/pubmed/17307905 .
  27. Wakayama S, Suetsugu R,Van Thuan N, Ohta H, Kishigami S, Wakayama T. Establishment of mouse embryonic stem cell lines from somatic cell nuclei by nuclear transfer into aged, fertilization-failure mouse oocytes. Curr Biol. 20; 17:120-121. 2007 (IF: 10.881).
    http://www.ncbi.nlm.nih.gov/pubmed/17307041
  28. Hikichi T, Wakayama S, Mizutani E, Takashima Y, Kishigami S,Van Thuan N, Ohta H, Thuy Bui H, Nishikawa S, Wakayama T. Differentiation potential of parthenogenetic embryonic stem cells is improved by nuclear transfer. Stem Cells. 2007 Jan;25(1):46-53 (IF: 7.9).
    http://www.ncbi.nlm.nih.gov/pubmed/17008422
  29. Mizutani E,Ohta H, Kishigami S, Van Thuan N, Hikichi T, Wakayama S, Kosaka M, Sato E, Wakayama T. Developmental ability of cloned embryos from neural stem cells. Reproduction. 2006 Dec;132(6):849-57 (IF: 3.413).
    http://www.ncbi.nlm.nih.gov/pubmed/1712774.
  30. Kishigami S,Bui HT, Wakayama S, Tokunaga K, Van Thuan N, Hikichi T, Mizutani E, Ohta H, Suetsugu R, Sata T, Wakayama T. Successful mouse cloning of an outbred strain by trichostatin A treatment after somatic nuclear transfer. J Reprod Dev. 2007 Feb;53(1):165-70 (IF: 1.46).
    http://www.ncbi.nlm.nih.gov/pubmed/17077581
  31. Satoshi Kishigami, Sayaka Wakayama,Van Thuan N, Hiroshi Ohta, Eiji Mizutani, Takafusa Hikichi, Hong-Thuy Bui, Sebastian Balbach, Atsuo Ogura, Michele Boiani and Teruhiko Wakayama. Production of cloned mice by somatic cell nuclear transfer. Nature, 1:125 – 138. 2006 (IF: 9.924).
    http://www.nature.com/nprot/journal/v1/n1/abs/nprot.2006.21.html
  32. Kishigami S, Mizutani E, Ohta H, Hikichi T,Van Thuan N, Wakayama S, Bui HT, Wakayama T. Significant improvement of mouse cloning technique by treatment with trichostatin A after somatic nuclear transfer. Biochem Biophys Res Commun.340:183-189. 2006 (IF: 2.523).
    http://www.ncbi.nlm.nih.gov/pubmed/16356478
  33. Bui HT,Van Thuan N, Wakayama T, Miyano T. Chromatin remodeling in somatic cells injected into mature pig oocytes. Reproduction. 131:1037-49. 2006 (IF: 3.413).
    http://www.ncbi.nlm.nih.gov/pubmed/16735543
  34. Wakayama, M. L. Jakt, M. Suzuki, R. Araki, T. Hikichi, S. Kishigami, H. Ohta,Van Thuan N, E. Mizutani, Y. Sakaide, S. Senda, S. Tanaka, M. Okada, M. Miyake, M. Abe, S. I. Nishikawa, K. Shiota, T. Wakayama. Equivalency of Nuclear Transfer-Derived Embryonic Stem Cells to those Derived from Fertilized Mouse Blastocyst. Stem Cells. 2006 (IF: 7.9).
    http://www.ncbi.nlm.nih.gov/pubmed/16690779
  35. Kishigami S, Wakayama S,Van Thuan N, Wakayama T. Cloned mice and embryonic stem cell establishment from adult somatic cell. Hum Cell. 19:2-10. 2006 (IF: 1.129).
    http://www.ncbi.nlm.nih.gov/pubmed/16643601
  36. Kishigami S, Hikichi T,Van Thuan N, Ohta H, Wakayama S, Bui HT, Mizutani E, Wakayama T. Normal specification of the extraembryonic lineage after somatic nuclear transfer. FEBS Lett. 580:1801-1806. 2006 (IF: 3.538).
    http://www.ncbi.nlm.nih.gov/pubmed/16513118.
  37. Tamada H,Van Thuan N, Reed P, Nelson D, Katoku-Kikyo N, Wudel J, Wakayama T, Kikyo N. Chromatin decondensation and nuclear reprogramming by nucleoplasmin. Mol Cell Biol. 26:1259-1271. 2006 (IF: 5.766).
    http://www.ncbi.nlm.nih.gov/pubmed/16449640
  38. Kishigami S,Van Thuan N, Hikichi T, Ohta H, Wakayama S, Mizutani E, Wakayama T. Epigenetic abnormalities of the mouse paternal zygotic genome associated with microinsemination of round spermatids. Dev Biol. 289:195-205. 2006 (IF: 4.407).
    http://www.ncbi.nlm.nih.gov/pubmed/16325170.
  39. Wakayama S, Mizutani E, Kishigami S,Van Thuan N, Ohta H, Hikichi T, Bui HT, Miyake M, Wakayama T. Mice cloned by nuclear transfer from somatic and ntES cells derived from the same individuals. J Reprod Dev. 51:765-72. 2005 (IF: 1.460).
    http://www.ncbi.nlm.nih.gov/pubmed/16227673
  40. Hikichi T, Kishigami S, Van Thuan N, Ohta H, Mizutani E, Wakayama S, Wakayama T. Round spermatids stained with mitotracker can be used to produce offspring more simply.Zygote. 13:55-61. 2005 (IF: 1.337).
    http://www.ncbi.nlm.nih.gov/pubmed/15984163
  41. Wakayama S, Kishigami S,Van Thuan N, Ohta H, Hikichi T, Mizutani E, Yanagimachi R, Wakayama T. Propagation of an infertile hermaphrodite mouse lacking germ cells by using nuclear transfer and embryonic stem cell technology. Proc Natl Acad Sci U S A.; 102:29-33. 2005 (IF: 10.50).
    http://www.ncbi.nlm.nih.gov/pubmed/15618395
  42. Mizutani E, Ohta H, Kishigami S,Van Thuan N, Hikichi T, Wakayama S, Sato E, Wakayama T.Generation of progeny from embryonic stem cells by microinsemination of male germ cells from chimeric mice. Genesis. 43:34-42. 2005 (IF: 2.864).
    http://www.ncbi.nlm.nih.gov/pubmed/16106362
  43. Wakayama S, Ohta H, Kishigami S,Van Thuan N, Hikichi T, Mizutani E, Miyake M, Wakayama T. Establishment of male and female nuclear transfer embryonic stem cell lines from different mouse strains and tissues. Biol Reprod. 72:932-936. 2005 (IF: 4.009).
    http://www.ncbi.nlm.nih.gov/pubmed/15601921 .
  44. Wakayama S,Van Thuan N, Kishigami S, Ohta H, Mizutani E, Hikichi T, Miyake M, Wakayama T. Production of offspring from one-day-old oocytes stored at room temperature. J Reprod Dev. 50:627-637. 2004 (IF: 1.460).
    http://www.ncbi.nlm.nih.gov/pubmed/15647614 .
  45. Kishigami S,Van Thuan N, Wakayama S, Hikichi T, Wakayama T. A novel method for isolating spermatid nuclei from cytoplasm prior to ROSI in the mouse. Zygote. 12:321-327. 2004 (IF: 1.337).
    http://www.ncbi.nlm.nih.gov/pubmed/15751541 .
  46. Satoshi Kishigami, Sayaka Wakayama,Nguyen Van Thuan and Teruhiko Wakayama.Similar Time Restriction for Intracytoplasmic Sperm Injection and Round Spermatid Injection into Activated Oocytes for Efficient Offspring Production. Biol Reprod; 70:1863–1869. 2004 (IF: 4.009).
    http://www.ncbi.nlm.nih.gov/pubmed/14985245 .
  47. Miyake M,Van Thuan N, Okada K, Katayama M, Kure-bayashi S, Harayama H. Early development of porcine parthenogenetic diploids in-vitro. In Reproductive Biotechnology and Relating Physiology. Miyamoto, H. and Manabe, N. eds. p285-295, 2001.    
  48. Van Thuan N, Wakayama S, Kishigami S, Wakayama T. Donor Centrosome Regulation of Initial Spindle Formation in Mouse Somatic Cell Nuclear Transfer: Roles of Gamma-Tubulin and Nuclear Mitotic Apparatus Protein 1.Biol Reprod. 74:777–787, 2006. (IF: 4.009).
    http://www.ncbi.nlm.nih.gov/pubmed/16407502

Published in International Books

  1. Sprinciples of Cloning (2013, ELSEVIER)
    Chapter 11 “Enhancing SCNT with Chromatin Remodeling Agents”
    Satoshi Kishigami, Van Thuan Nguyen, Sayaka Wakayama, Teruhiko Wakayama
    ISBN: 978-0-12-386541-0
    http://www.sciencedirect.com/science/book/9780123865410
  1. Advanced Protocols for Animal Transgesis (2011, Springer)
    Chapter 13 “Mouse Cloning by Nuclear Transfer”
    Sayaka Wakayama, Nguyen Van Thuan, Teruhiko Wakayama
    ISBN 978-3-642-20791-4
    http://www.springer.com/us/book/9783642207914

Recent invited speaker at International conference

  1. Nguyen Van Thuan. Induced Pluripotent Stem Cell by GV extract: gviPS Cells. The 10th Annual Meeting of Asian Reproductive Biotechnology Society, August 19-25, 2013. Mui ne, Phan Thiet, Vietnam. (Invited speaker).
  2. Nguyen Van Thuan. Advances in Cloning of Animals and Therapeutic Cloning. The 1st gene and Immunotherapy conference. March 21-22, 2013. Hochiminh City, Vietnam. (Invited speaker).
  3. Nguyen Van Thuan. Reprogramming of somatic cell by GV oocytes extract: gviPS cells. The 9th Annual Meeting of Asian Reproductive Biotechnology Society, Oct 23-28, 2012. Manila,Philippines (Invited speaker).
  4. Nguyen Van Thuan. The mechanisms involved in enhancement of somatic cell reprogramming by HDACi. The 8th Annual Meeting of Asian Reproductive Biotechnology Society, Oct 25-30, 2011, Guilin, China. (Invited speaker).
  5. Nguyen Van Thuan. From somatic cells to gviPS cells and full-term. The 7th Annual Meeting of Asian Reproductive Biotechnology Society, Nov 8-10, 2010, Kuala Lumpur, Malaysia. (Invited speaker).
  6. Nguyen Van Thuan. Somatic cells reprogramming: from somatic cell to iPS cells and full-term development. 2010 Annual Coference of Korea Society of Animal Reproduction, June 18, 2010, Konkuk University, Seoul Korea, (Invited speaker).
  7. Nguyen Van Thuan. Histone deacetylase inhibitor scriptaid and the development of cloning animal. The 6th Annual Conference of Asian Reproductive Biotechnology Society. Novemner 14-19, 2009. Seam Reap, Cambodia, (Invited speaker).
  8. Nguyen Van Thuan. Histone deacetylase inhibitor and development of cloning animal. National Advance Reproductive Biotechnology Workshop of Malaysia, October 19-23, 2009. Thistle Port Dickson Resort & Agro-Biotechnology Institute, Malaysia. (Invited speaker).
  9. Nguyen Van Thuan. Advances in Reproductive Biotechnology and applications of ICSI and SCNT. The first international conference on Reproductive Biotechnology, Mahidol University, Bangkok, Thailand, Jan, 2009 (Invited speaker).
  10. Nguyen Van Thuan. New advances in somatic cell nuclear transfer in mouse and porcine. The 5th Annual Conference of Asian Reproductive Biotechnology Society. Kunming, Yunam, China. November 27-30, 2008. (Invited speaker).
  11. Nguyen Van Thuan. Somatic nuclear repmodeling and reprogramming: New advances in somatic cell nuclear transfer. International symposium “Recent Progresses in Animal Biotechnology”. Research Center for Transgenic Cloned Pigs Division of Animal Science & Resources, Chungnam National University. Oct 17-18, 2008. (Invited speaker) 
  12. Nguyen Van Thuan. Rescue of full-term development in “unclonable” inbred mouse strains. The 4th Annual Conference of Asian Reproductive Biotechnology Society. National University of Singapore, Singapore. November 24-28, 2007. (Invited speaker).
  13. Nguyen Van Thuan. Somatic nuclear remodeling and reprogramming in mouse cloned embryo. The 3rd Korea-Japan Joint Symposium of Animal Reproductive Biology and Biotechnology. The University of Tokyo, Japan. Oct 18-19, 2007. (Invited speaker).
  14. Nguyen Van Thuan. Characterization of Somatic Cell Nuclear Remodeding and Reprogramming in Cloned Mouse Embryos. The 7th International Symposium on Developmental Biotechnology. Cheju, Korea. Oct 26-27, 2007. (Invited speaker).
  15. Nguyen Van Thuan, Sayaka Wakayama, Satoshi Kishigami, Hiroshi Ohta, Takafusa Hikichi, Eiji Mizutani, Hong-Thuy Bui, Teruhiko Wakayama. Injection of somatic cell cytoplasms into oocytes before ICSI impaired full-term development and increased placenta weight in mice. 32st Annual Conference of the International Embryo Transfer Society. Florida, America. January 7-10, 2006.
  16. Nguyen Van Thuan. Characterization of Somatic nuclear Remodeling and Reprograming in mouse cloned embryo. The 3rd Annual Conference of the Asian Reproductive Biotechnology Society. Horizon Hotel, Hanoi, Vietnam. Nov 29 – Dec 3, 2006. (Invited speaker)
  17. Nguyen Van Thuan, Sayaka Wakayama, Satochi Kishigami, Teruhiko Wakayama. New preservation method for mouse spermatozoa without freezing. The 2nd Annual Conference of the Asian Reproductive Biotechnology, Bangkok, Thailand. November 2-7, 2005. (Invited Speaker)
  18. Nguyen Van Thuan, Wakayama S, Kishigami S, Hikichi T, Ohta H, Mizutani E, Hong-Thuy B, and Wakayama T. First cleavage plane is defined in the mouse zygote by the tie of female pronucleus and the second polar body through tie-microtubule cluster. The 98th Annual Meeting of Japanese Society of Animal Reproduction. 14-16 September, Shizuoka, Japan 2005.
  19. Nguyen Van Thuan, Sayaka Wakayama, Hong-Thuy Bui, and Teruhiko Wakayama. Active methylation and acetylation of histone H3-K9 in mouse embryo with different proportions of maternal and paternal genome. 31st Annual Conference of the International Embryo Transfer Society. Copenhagen, Denmark. January 9-11, 2005.
  20. Nguyen Van Thuan. Spindle morphogenesis and effects of in vitro remodeling of donor somatic cell on the developmental viability in mouse cloning. The 1st Asian Reproductive Biotechnology Society conference. The University of Agriculture and Forestry of Hochiminh City, Vietnam. April 12-14, 2004 (Invited speaker)
  21. Nguyen Van Thuan, Sayaka Wakayama, Satoshi Kishigami, Hiroshi Ohta, Takafusa Hikichi, Eiji Mizutani, and Teruhiko Wakayama. Different mutation of DNA methyltransferases, histone H3 Lysine 9 methylation and acetylation during preimplantational development in mouse ICSI, somatic cell and ES cell nuclear transfer embryos. The 13th conference of the International Society of Differentiation. Waikiki, Honolulu, Hawaii. 5-9 September, 2004.
  22. Nguyen Van Thuan, Sayaka Wakayama, Satoshi Kishigami, Hiroshi Ohta, Takafusa Hikichi, Eiji Mizutani, and Teruhiko Wakayama. Effects of treatments of donor somatic cells on the efficiency of mouse cloning. The 37th Annual meeting of society for the study of reproduction. August 1-4, 2004, University of British Columbia, Vancouver, British Columbia, Canada.
  23. Nguyen Van Thuan, Sayaka Wakayama, Satoshi Kishigami, Teruhiko Wakayama. Alterations of histone H3 methylation, acetylation and phosphorlation at interphase chromatin and metaphase chromosome during the early development of mouse embryos. The Annual Meeting of Japanese Society of Animal Reproduction. 11-13 September 2004, Obihiro, Japan. Hiroshima University, Hirosima.
  24. Nguyen Van Thuan. Spindle morphogenesis and effects of in vitro remodeling of donor somatic cell on the developmental viability in mouse cloning. The 1st Asian Reproductive Biotechnology workshop. 12-14 April 2004, Nong Lam University, Hochiminh, Vietnam. (Invited Speaker).
  25. Nguyen Van Thuan, Sayaka Wakayama, Satoshi Kishigami and Teruhiko Wakayama. Spindle morphogenesis and the morphology of chromosome in mouse nuclear transfer: an abnormal stage in cloning of mice. The 30th Annual conference of the international embryo transfer society, Portland, Oregon, 10-14 January 2004.
  26. Nguyen Van Thuan, Sayaka Wakayama, Satoshi Kishigami and Teruhiko Wakayama. Spindle morphogenesis in mouse nuclear transfer: an abnormal start of reprogramming in cloning? The 96th Annual Meeting of Japanese Society of Animal Reproduction. 11-13 September, Obihiro, Japan 2003.
  27. Nguyen Van Thuan, Sayaka Wakayama, Satoshi Kishigami and Teruhiko Wakayama. Normal fertilization and development of oocytes injected with long-term preserved mouse spermatozoa without freezing. The 36th Annual Meeting of the Society for the Study of Reproduction (SSR)19-22 July, 2003 Cincinnati, Ohio, America.
  28. Nguyen Van Thuan, Sayaka Wakayama, Satoshi Kishigami and Teruhiko Wakayama. Long-term preservation of mouse spermatozoa without freezing: effect of temperature and osmolarity. The 101th Annual Meeting of Japanese Society of Animal Science. 27-29 March, 2003, Stukuba, Japan.
  29. Nguyen Van Thuan, Teruhiko Wakayama, Bui Hong Thuy, Hiroshi Harayama, Takashi Miyano, and Masashi Miyake. The presence of non-polar essential amino acids in a medium before the 4-cell stage disrupts actine filament distribution in porcine parthenogenetic diploids. The 29th Annual conference of the international embryo transfer society, Auckland, New Zealand, 11-15 January, 2003.